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(Deoxyribonucleic acid) DNA was demonstrated as the genetic material by Oswald Theodore Avery in 1944. Its double helical strand structure composed of four bases was determined by James D. Watson and Francis Crick in 1953, leading to the central dogma of molecular biology. In most cases, genomic DNA defined the species and individuals, which makes the DNA sequence fundamental to the research on the structures and functions of cells and the decoding of life mysteries [1]. DNA sequencing technologies could help biologists and health care providers in a broad range of applications such as molecular cloning, breeding, finding pathogenic genes, and comparative and evolution studies. DNA sequencing technologies ideally should be fast, accurate, easy-to-operate, and cheap. In the past thirty years, DNA sequencing technologies and applications have undergone tremendous development and act as the engine of the genome era which is characterized by vast amount of genome data and subsequently broad range of research areas and multiple applications. It is necessary to look back on the history of sequencing technology development to review the NGS systems (454, GA/HiSeq, and SOLiD), to compare their advantages and disadvantages, to discuss the various applications, and to evaluate the recently introduced PGM (personal genome machines) and third-generation sequencing technologies and applications. All of these aspects will be described in this paper. Most data and conclusions are from independent users who have extensive first-hand experience in these typical NGS systems in BGI (Beijing Genomics Institute).
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BGI is establishing their cloud services. Combined with advanced NGS technologies with multiple choices, a plug-and-run informatics service is handy and affordable. A series of softwares are available including BLAST, SOAP, and SOAP SNP for sequence alignment and pipelines for RNAseq data. Also SNP calling programs such as Hecate and Gaea are about to be released. Big-data studies from the whole spectrum of life and biomedical sciences now can be shared and published on a new journal GigaSicence cofounded by BGI and Biomed Central. It has a novel publication format: each piece of data links to a standard manuscript publication with an extensive database which hosts all associated data, data analysis tools, and cloud-computing resources. The scope covers not just omic type data and the fields of high-throughput biology currently serviced by large public repositories but also the growing range of more difficult-to-access data, such as imaging, neuroscience, ecology, cohort data, systems biology, and other new types of large-scale sharable data.
Abstract:This research analyses the differences in impact assessment results depending on the choice of a certain software-database combination. Six packaging systems were modelled in three software-database combinations (GaBi database in GaBi software, ecoinvent 3.6 database in openLCA, Environmental Footprint database in openLCA). The chosen Life Cycle Impact Assessment (LCIA) method is EF 2.0. Differences and errors in the implementation of the LCIA method are a possible source of deviations. We compared the published characterisation factors with the factors implemented in the software-database combinations. While results for the climate change category are similar between the different databases, this is not the case for the other impact categories. In most cases, the use of the ecoinvent 3.6 database leads to higher results compared to GaBi. This is partly due to the fact, that ecoinvent datasets often include more background processes than the corresponding GaBi datasets. We found striking discrepancies in LCIA implementation, including the lack of regionalisation for water use in ecoinvent. A meaningful communication of LCIA results requires an excellent knowledge of the analysed product system, as well as of database quality issues and LCIA methodology. We fully acknowledge the constant efforts of database providers to improve their databases.Keywords: packaging; life cycle assessment; databases; life cycle impact assessment; ecoinvent; GaBi; environmental footprint database
49. As silence and careful listening disappear, replaced by a frenzy of texting, this basic structure of sage human communication is at risk. A new lifestyle is emerging, where we create only what we want and exclude all that we cannot control or know instantly and superficially. This process, by its intrinsic logic, blocks the kind of serene reflection that could lead us to a shared wisdom.
67. The parable eloquently presents the basic decision we need to make in order to rebuild our wounded world. In the face of so much pain and suffering, our only course is to imitate the Good Samaritan. Any other decision would make us either one of the robbers or one of those who walked by without showing compassion for the sufferings of the man on the roadside. The parable shows us how a community can be rebuilt by men and women who identify with the vulnerability of others, who reject the creation of a society of exclusion, and act instead as neighbours, lifting up and rehabilitating the fallen for the sake of the common good. At the same time, it warns us about the attitude of those who think only of themselves and fail to shoulder the inevitable responsibilities of life as it is.
68. The parable clearly does not indulge in abstract moralizing, nor is its message merely social and ethical. It speaks to us of an essential and often forgotten aspect of our common humanity: we were created for a fulfilment that can only be found in love. We cannot be indifferent to suffering; we cannot allow anyone to go through life as an outcast. Instead, we should feel indignant, challenged to emerge from our comfortable isolation and to be changed by our contact with human suffering. That is the meaning of dignity.
79. The Samaritan who stopped along the way departed without expecting any recognition or gratitude. His effort to assist another person gave him great satisfaction in life and before his God, and thus became a duty. All of us have a responsibility for the wounded, those of our own people and all the peoples of the earth. Let us care for the needs of every man and woman, young and old, with the same fraternal spirit of care and closeness that marked the Good Samaritan.
89. Nor can I reduce my life to relationships with a small group, even my own family; I cannot know myself apart from a broader network of relationships, including those that have preceded me and shaped my entire life. My relationship with those whom I respect has to take account of the fact that they do not live only for me, nor do I live only for them. Our relationships, if healthy and authentic, open us to others who expand and enrich us. Nowadays, our noblest social instincts can easily be thwarted by self-centred chats that give the impression of being deep relationships. On the contrary, authentic and mature love and true friendship can only take root in hearts open to growth through relationships with others. As couples or friends, we find that our hearts expand as we step out of ourselves and embrace others. Closed groups and self-absorbed couples that define themselves in opposition to others tend to be expressions of selfishness and mere self-preservation.
167. Education and upbringing, concern for others, a well-integrated view of life and spiritual growth: all these are essential for quality human relationships and for enabling society itself to react against injustices, aberrations and abuses of economic, technological, political and media power. Some liberal approaches ignore this factor of human weakness; they envisage a world that follows a determined order and is capable by itself of ensuring a bright future and providing solutions for every problem.
176. For many people today, politics is a distasteful word, often due to the mistakes, corruption and inefficiency of some politicians. There are also attempts to discredit politics, to replace it with economics or to twist it to one ideology or another. Yet can our world function without politics? Can there be an effective process of growth towards universal fraternity and social peace without a sound political life?[157]
191. At a time when various forms of fundamentalist intolerance are damaging relationships between individuals, groups and peoples, let us be committed to living and teaching the value of respect for others, a love capable of welcoming differences, and the priority of the dignity of every human being over his or her ideas, opinions, practices and even sins. Even as forms of fanaticism, closedmindedness and social and cultural fragmentation proliferate in present-day society, a good politician will take the first step and insist that different voices be heard. Disagreements may well give rise to conflicts, but uniformity proves stifling and leads to cultural decay. May we not be content with being enclosed in one fragment of reality.
204. There is a growing conviction that, together with specialized scientific advances, we are in need of greater interdisciplinary communication. Although reality is one, it can be approached from various angles and with different methodologies. There is a risk that a single scientific advance will be seen as the only possible lens for viewing a particular aspect of life, society and the world. Researchers who are expert in their own field, yet also familiar with the findings of other sciences and disciplines, are in a position to discern other aspects of the object of their study and thus to become open to a more comprehensive and integral knowledge of reality.
212. If something always serves the good functioning of society, is it not because, lying beyond it, there is an enduring truth accessible to the intellect? Inherent in the nature of human beings and society there exist certain basic structures to support our development and survival. Certain requirements thus ensue, and these can be discovered through dialogue, even though, strictly speaking, they are not created by consensus. The fact that certain rules are indispensable for the very life of society is a sign that they are good in and of themselves. There is no need, then, to oppose the interests of society, consensus and the reality of objective truth. These three realities can be harmonized whenever, through dialogue, people are unafraid to get to the heart of an issue. 2ff7e9595c
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